Figure 3. Expressions of TLR1, TLR2, TLR3, TLR4, MD-2, TLR5, TLR6, TLR7, TLR8, TLR9, MyD88, NOD1, and NOD2 in human gingival fibroblasts, detected by immunostaining. Fibroblasts were cultured until confluent at 37°C. After fixation, the cells were treated with anti-TLR2, anti-TLR3, anti-TLR4, anti-TLR5, anti-TLR6, anti-TLR7, anti-TLR8, anti-TLR9, anti-MyD88, anti-NOD1, and anti-NOD2 antibodies and then visualized with Alexa Fluor 488 (green). The nuclei were visualized by being stained with 4',6-diamino-2-phenylindole (blue). Scale bars: 20 µm. The results are representative of 3 different experiments demonstrating similar results.