Figure 1. Expressions of TLR1, TLR2, TLR3, TLR4, MD-2, TLR5, TLR6, TLR7, TLR8, TLR9, MyD88, NOD1, and NOD2 mRNA in human gingival fibroblasts. Fibroblasts were cultured until confluent at 37°C. After incubation, the total RNA was extracted, and the mRNA expressions of TLR1, TLR2, TLR3, TLR4, MD-2, TLR5, TLR6, TLR7, TLR8, TLR9, MyD88, NOD1, and NOD2 were analyzed with PCR, with the primers shown in (a). The clear expressions of these mRNAs were detected (b). The results presented are representative of 3 different experiments demonstrating similar results.