Figure 2. Effects of IL-1 on the phosphorylation of ERK1/2, p38, and JNK in odontogenic keratocyst fibroblasts. Fibroblasts (4 x 10⁴ cells/cm²) were incubated with 0.1 nM rhIL-1 for various intervals (A,B,C), or with various concentrations of rhIL-1 for 10 min (D,E) or 20 min (F) at 37°C. Western immunoblotting for phospho-ERK1/2 (A,D), phospho-p38 (B,E), and phospho-JNK (C,F) was performed as described in "MATERIALS & METHODS". The values were normalized to the amounts of the control. Vertical bars indicate mean ± SD (n = 3). * Significant difference at p < 0.05.