Figure 1. hCMV antigen expression in epithelial cells. HeLa (A) and primary cells (B) were infected with hCMV for 48 hrs. Cells were fixed with ice-cold absolute methanol and blocked with 10% normal goat serum. To detect hCMV IE antigens, we incubated cells with primary mouse antibodies (1:80) recognizing IE (clone E13) (Argene, Varilhes, France), and reacted them with fluorescein-conjugated goat anti-mouse IgG antibodies (Dako, Heverlee, Belgium) at 1:200 dilution. Images were made with an Olympus IX70 confocal microscope. (1) Phase-contrast image, (2) fluorescent image, (3) overlap image showing bright IE-positive nuclei for some epithelial cells.