Figure 3. Quantitative RT-PCR analysis for Igf2. The relative expression of Igf2 from unchallenged placenta (n = 6) and from growth-restricted challenged placenta (n = 6) was compared. Reactions were performed 2 independent times in triplicate. Mean values and error bars are presented. Statistical analysis was performed with one-way ANOVA. There was a significant (p = 0.0005) 2.3-fold decrease in the relative expression of Igf2 in the challenged placenta at E16.5.