Figure 3. N-terminal sequence characterization of rpAMBN polypeptides generated by rpMMP-20 digestion. The rpAMBN digestions after 0.5 and 4 hrs were separated on 4–12% NuPage gels, transblotted to a membrane, and lightly stained with CBB; 7 strips of membrane were analyzed by N-terminal sequencing. CBB-stained replica gels of the ones used for this analysis are shown here. Arrowheads indicate the positions of the bands excised for analysis. The band labels (i.e., S65, etc.) indicate the apparent molecular weight of the excised protein. The numbers at the start of the protein sequences indicate the number of the first amino acid in the 365-amino-acid porcine AMBN sequence. When more than one sequence was obtained for a single strip of membrane, the separate sequences are indicated by a, b, or c.