Figure 4. Radii R_s and R_h (A), numbers of protein molecules N per particle (B), and apparent protein densities d of particles of rM179 (C), as measured by means of small-angle x-ray scattering (SAXS) and dynamic light scattering (DLS) during heating and subsequent cooling of the protein solution (2 mg/mL, pH 8). Heating the sample led to an irreversible increase of the hydrodynamic radius and number of protein molecules per particle, along with a decrease of the apparent density evaluated from DLS data. In contrast, the corresponding parameters measured by means of SAXS showed only a comparatively weak effect. (Reprinted from Aichmayer et al., 2005, with permission from Elsevier.)