Figure 3. RT-PCR analysis of Biostite^®-modulated genes. Plasminogen activator inhibitor-1 (PAI1), tenascin, and osteonectin RNA levels in MG-63 cells exposed for 48 hrs to 500 µg/mL of Biostite^® (+). RT-PCR products were subjected to electrophoresis on 2% agarose gel and visualized by UV exposure. A representative analysis is shown on the left. The level of specific bands was normalized for the ß-actin cDNA content, and the results from three experiments are shown on the right as mean values ± SEM of controls (open bars) and treated cells (patterned bars) (*P < 0.05; **P < 0.01; ***P < 0.001, ANOVA). Similar results have been obtained by normalization with respect to GAPDH cDNA (data not shown).