Figure 2. Genes differentially expressed in response to Biostite^®. MG-63 cells (3 x 10⁴/mL) were seeded in T175 flasks and cultured for 48 hrs with and without Biostite^® (500 µg/mL) (n = 3 for treated and control cells). RNA extraction, purification, ³²P-labeled cDNA synthesis and hybridization conditions were detailed in the METHODS section and in the APPENDIX. Expressed genes in Cancer (black bars) and Cell interaction (patterned bars) arrays are defined as up-regulated and down-regulated when expression levels showed more than a two-fold increase and reduction, respectively, after treatment, compared with untreated controls. Relative values were averaged for the results obtained in two hybridization experiments (see METHODS/APPENDIX for technical details). The number of genes affected by Biostite^® is shown with respect to the expressed genes which were detected in each array (inset).