Figure 1. The effects of silibinin on cell invasion, motility, and cell-matrix adhesion of SCC-4 cells. Cells were treated with silibinin at a concentration of 0, 25, 50, 75, or 100 µM for 24 hrs, and were then subjected to analyses for invasion (A), motility (B), and cell-matrix adhesion (C) as described in MATERIALS & METHODS. Data represent the mean ± SD of at least 3 independent experiments. Statistical significance was determined by Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001).