Figure 2. Densitometry analysis of immunocytochemistry photographs (a) n = 5 photos from each sample collected from all rats in each experimental group) for iNOS, PAR, and nitrotyrosine from gingivomucosal tissue was assessed. The assay was carried out with Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Evans blue content (b) and myeloperoxidase (MPO) activity (c) in gingivomucosal tissue were significantly increased by ligature compared with the contralateral side. Rosiglitazone (10 mg/kg i.p., daily for 8 days) significantly reduced Evans blue content and myeloperoxidase activity levels. Densitometry data are expressed as % of total tissue area. Data are means of mean ± SEM from n = 10 rats for each group. *P < 0.01 vs. non-ligated. °P < 0.01 vs. ligated.