Figure 3. Two-dimensional gel electrophoresis of dental biofilm formed in the absence (A) and presence (B) of sucrose (20 µg of proteins). Isoelectric focusing with pH range 4–7 and PAGE (8–18%). The gels were silver-stained. Spots were excised for in-gel digestion and analyzed by mass spectrometry (MALDI-TOF). The fingerprints were analyzed in Mascot and Prospector program with the NCBInr database. Arrows show calcium-binding proteins present only in biofilm formed in the absence of sucrose (A) when compared with biofilm formed in the presence of sucrose (B).