Figure 3. Time courses of pilocarpine-induced water intakes and salivary secretion from the parotid gland. (A) The time courses of water intake by the intraperitoneal () IP, 12 µmol/kg, n = 6) and intracerebroventricular injection of pilocarpine(•) ICV, 0.3 nmol, n = 6). There was a significant difference at 15 min between the intraperitoneal and intracerebroventricular injections (Bonferroni post-test followed by two-way ANOVA; *P < 0.05). (B) The time of the start of increased water intake induced by the intraperitoneal and intracerebroventricular injection of pilocarpine (open bar, 12 µmol/kg; filled bar, 0.3 nmol). **P < 0.01 by unpaired t test. (C) The time courses of parotid salivary secretion induced by the intraperitoneal injection of pilocarpine at 12 µmol/kg without (), n = 6) and with the intracerebroventricular pre-injection of atropine at 1 nmol (), n = 5). The time courses were not significantly different by two-way ANOVA.