Figure 2. Effects of intracerebroventricularly (ICV) injected pilocarpine on water intake and salivary secretion from the parotid gland. The water intake (A) for 60 min increased dose-dependently, but the salivary secretion (B) did not. The increased water intake by the intracerebroventricular injection of pilocarpine at 30 nmol was suppressed by the intracerebroventricular pre-injection of atropine (ATR) at 1 nmol. The results are represented as means ± SEM. The numbers of rats tested were between 5 and 7. n.s. = not significant. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the control saline group. +++P < 0.001 vs. the ATR group.