Figure 4. OPG expression in dental follicle cells transfected with CSF-1 targeting siRNA. Result in Panel B is the mean of 3 separate experiments. (Panel A) Semi-quantitative RT-PCR showing greater OPG expression after 48 and 72 hrs of transfection, in comparison with the controls at those times. (Panel B) Real-time RT-PCR results showing relative gene expression with controls (0 nM) set to 1 at each time point indicated. At both 48 and 72 hrs, the OPG gene expression is significantly increased (*) after transfection with 80 nM of CSF-1 siRNA (P 0.05), by Student’s t test. Relative gene expression was calculated by the Delta C_T method, which was normalized to the endogenous control of ß-actin. Error bars represent SDs.