Figure 2. Fibroblasts from patients undergoing CsA-immunosuppressive therapy without any clinical signs of gingival overgrowth (noGO). (a,b) Immunocytochemistry of PLCß₁ at the TEM level of FCS-deprived (2a; UN = untreated cells; HC = heterochromatin; IC = interchromatin; N = nucleolus; bar = 0.5 µm) and CsA-treated cells (2b; +CsA; bar = 0.5 µm). The analysis was performed on thin sections of cell monolayers, partially dehydrated, and embedded in LR White resin at low temperature, a procedure that ensures a reliable antigen detection. Due to the absence of osmium fixation and to the characteristics of the hydrophilic resin, the membranes are not electrondense. (c) Quantitative evaluation of the nuclear labeling density was performed with the Mann-Whitney U test (UN; n = 10) (+CsA; n = 10). Values are the means of examined samples ± SD. No significant difference was observed (power = 0.74).