Figure 4. Inhibition of phenol-water extract binding to immobilized CD14 by T. medium glycoconjugate. Biotinylated phenol-water extract or E. coli LPS with or without T. medium glycoconjugate were added to CD14- or BSA-coated wells in the presence or absence of LPS-binding protein. The binding of biotinylated phenol-water extract or E. coli LPS was detected with the use of HRP-conjugated streptavidin. Representative results from 3 independent experiments are presented.