Figure 1. CD14 expression and IL-8 production in human gingival fibroblasts. (A) Cell-surface expression of CD14 on human gingival fibroblasts was determined with a specific antibody (bold line) or its isotype control (thin line), as described in MATERIALS & METHODS. (B) IL-8 production in human gingival fibroblasts stimulated with various periodontopathic bacterial phenol-water extracts. The cells were stimulated with the indicated doses of the phenol-water extracts or with E. coli LPS in the presence of 5% FBS. The results are shown as the mean ± SEM. Representative results from 3 independent experiments are presented.