Figure 1. Time course of angiogenesis in oral mucosal and skin wounds. The vascular density of the wounds on days 1, 3, 5, and 7 after injury was determined by image analysis of CD31-stained tissues. Data are expressed as mean ± SE (n = 5). Scale bars (lower right corner of each panel): 200 µm. (A) Immunohistochemical localization of vessels in the wound bed was performed with anti-CD31 antibody. The photographs depict immunostained histologic sections of wounds from days 5 and 7 after wounding occurred. Original magnification x50. (B) Vessel density in normal skin and wound bed. The % vascularization [(CD31-positive area/Total wound bed area) x 100] was determined for 2 wound sections from each of 5 mice. (C) Increase in vessel density in wound bed over the baseline (normal tissue vasculature). We calculated the change in vascularity by dividing the vasculature value of each day by baseline value. Data are expressed as mean ± SE (n = 5). p < 0.001, mucosa vs. skin. NS, normal skin.