Figure 4. Gelatinolytic activities of the cultured tissue supernatants were studied with zymography. (A) Representative results from non-stimulated (–) and TNF--stimulated (+) healthy tissue sample supernatants at 48 hrs. (B) Inflamed tissue without (–) and with (+) TNF- blocker showed reverse findings at 48 hrs. Molecular-weight standards (kDa) are marked to the left (7.5% acrylamide separating gel containing 1 mg/mL gelatin).