Figure 3. LPS induces IL-8 expression in DPSCs. (A) DPSCs were treated with TNF or P. gingivalis LPS for the indicated times. Fifty-µg aliquots of protein extracts were probed with polyclonal antibodies against phospho-specific p65. For loading control, the membrane was stripped and re-probed with polyclonal antibodies against p65. (B) DPSCs were treated with TNF for the indicated times. Ten-µg aliquots of total RNAs were hybridized with ³²P-labeled IL-8 cDNA probes. For loading control, the blot was stripped and re-probed with ³²P-labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA probes. (C) DPSCs were treated with P. gingivalis LPS for the indicated times. Northern blot analysis was performed as described in (B). (D) DPSCs were treated with TNF for the indicated times. Total RNAs were probed with ³²P-labeled IL-6 cDNA probes. (E) DPSCs were treated with E. coli LPS for the indicated times. Total RNAs were probed with ³²P-labeded IL-6 cDNA probes.