Figure 2. TNF or LPS induces the nuclear translocation of NF-B. (A) DPSCs were treated with TNF (10 ng/mL) for the indicated time. Five-µg aliquots of nuclear proteins were incubated with ³²P-labeled NF-B oligonucleotide probes. For the supershift assay, nuclear proteins were pre-incubated with polyclonal antibodies against p65 for 10 min and then probed with³²P-labeled NF-B oligonucleotide probes. (B) DPSCs were treated with P. gingivalis LPS (1 µg/mL) for the indicated times. EMSA was performed as described in (A).