Figure 2. (A) Effects of doses of DSP and DPP on the release of neutrophil chemotactic factor derived from macrophages stimulated by DSP (DSP-M) and DPP (DPP-M). Macrophages were incubated for 1 hr with DSP and DPP (0.3, 1, and 10 µg/mL). The cells were then washed and incubated for 6 hrs in RPMI medium, and the supernatant was injected i.p. for evaluation of neutrophil migration. (B) Inhibitory activity of dexamethasone (DEX) on the release of neutrophil chemotactic factor derived from macrophages stimulated by DSP (DSP-M) and DPP (DPP-M). Macrophages were pre-treated with dexamethasone (10 µM/well) before stimulation with DSP and DPP (10 µg/mL). (C) Inhibitory effect of mast cell (MC) supernatant on neutrophil migration induced by DSP-stimulated macrophages (DSP-M). MC cultures were treated with DSP (10 µg/mL) and PBS for 1 hr, and the supernatant was used to treat macrophages before DSP stimulation. (D) Effects of anti-IL-4 and anti-IL-10 antibodies on inhibitory activity of DSP-stimulated MC supernatant (DSP-MC supernatant). DSP-MC supernatants were incubated with PBS, anti-IL-4, and anti-IL-10 antibodies (5 µg/mL) and then were used to treat macrophages before DSP stimulation. For all experiments, neutrophil migration was evaluated after 6 hrs. Results are mean ± SEM of 10 mice per group. The experiment was repeated twice. *P < 0.05 compared with RPMI; ^#P < 0.05 compared with the group without DEX (B); ^#P < 0.05 compared with the groups treated with PBS (C,D).