Figure 3. (a) Dye terminator chemistry method. Arrows indicate where translation starts. (b) Autoradiographic detection method. Arrows indicate where translation starts. ‘N’ marks the AG mutation in the initiation codon. (c) Restriction-enzyme analysis of exon 1 of PAX9 in the family. The longer fragment represents the mutated, uncleaved allele, and the shorter fragment the wild-type allele. The numbers indicate: (1) affected grandfather, (2) unaffected mother, (3) H₂O, (4) proband, and (5) affected father.