Figure 4. Immunohistochemical staining at week 8. (a) Anti-CD3 staining of the control group. (b) Anti-CD3 staining of the 20 mg/kg/day group. (c) Anti-CD25 staining of the control group. (d) Anti-CD25 staining of the 20 mg/kg/day group. Scale bars = 100 µm. (e) CD3+ cell-counts in the lesions of each group (n = 4). (f) CD25+ cell-counts in the lesions of each group (n = 4). (g) ED1+ cell-counts in the lesions of each group (n = 4). (h) TRAP staining of the control group. (i) TRAP staining of the 20 mg/kg/day group. Values are the means ± SD. *p < 0.05. **A value of p < 0.01 was determined as statistically different in comparison with controls.