Figure 2. CCL5 and CCL3 production by THP-1. (A) Cells were incubated in medium alone or stimulated with E. coli (Ec) or P. gingivalis (Pg) LPS for 24 hrs. Cells were either left untreated or pre-incubated with IL-10 or Dex 24 hrs before stimulation. (B) Cells were incubated with or without E. coli (Ec) or P. gingivalis (Pg) LPS (Primary stimulation) and then restimulated or not 24 hrs later with Ec.LPS or Pg.LPS (Secondary stimulation). Cell-free supernatants were analyzed by ELISA for CCL3 (
) and CCL5 (
) concentration. Data are expressed as means ± SD (n = 3). *p < 0.05.