Figure 2. Comparison between the patterns of Notch2 (D), nestin (E), and N-cadherin (F) immunostaining in human dental tissues after dental injury. (A) Schematic illustration showing reparative dentin production (asterisk) after a cavity preparation in an incisor. The blue line indicates an equivalent wounding area of a premolar shown in panel B. (B) Hematoxylin-eosin staining showing reparative dentin production (asterisk) 9 wks after class V cavity preparation (About et al., 2000). The deep blue line indicates an equivalent area shown in panel C. (C) Hematoxylin-eosin staining. Higher magnification of the reparative dentin area (asterisk) (Mitsiadis et al., 2003). (D-F) Immunostaining showing expression of Notch2 protein in sub-odontoblastic cells and blood vessels (Mitsiadis et al., 2003) (D), nestin protein in odontoblasts (About et al., 2000) (E), and N-cadherin in newly formed odontoblasts (Heymann et al., 2002) (F), after cavity preparation. Abbreviations: ab, alveolar bone; c, cementum; cav, cavity; d, dentin; e, enamel; nfo, newly formed odontoblasts; o, odontoblasts; oe, oral epithelium; p, pulp; rd, reparative dentin; soc, sub-odontoblastic cells. Scale bars: 50 µm. Figs. 2B and 2E are reprinted from Am J Pathol 157:287–295, 2000, with permission from the American Society for Investigative Pathology. Fig. 2F is reprinted from Am J Pathol 160:2123–2133, 2002, with permission from the American Society of Investigative Pathology. Figs. 2C and 2D is reprinted from Exp Cell Res 228:101–109 (Mitsiadis et al., "Notch 2 protein...", 2003), with permission from Elsevier.