Figure 3. Effects of GTF-S and dextran T10 on sucrose-dependent cell adherence. S. sanguinis ATCC10556, S. oralis ATCC10557, and S. gordonii ATCC10558 were used to prepare WSG-synthesizing GTF-S. Culture supernatants were collected, precipitated with 60% saturated ammonium sulfate, and dialyzed against 10 mM KPB. Each of the crude GTF-S enzyme solutions was added to a culture of S. mutans mutants B61 (A) and B42-21 (B). The effect of dextran T10 as a water-soluble glucan on adherence was also examined (C). Dextran T10 was added to the cultures of S. mutans mutants B61 and B42-21 to final concentrations of 0 to 4.0 mg/mL, and the same adhesion test was performed. Values are expressed as the mean ± standard deviation of triplicate experiments. Significant differences (*P < 0.05) between the GTF- or dextran-added group and non-added control are indicated.