Figure 1. Extracellular divalent cation inhibition of Hst-5-induced killing of C. albicans. C. albicans cells were incubated with 31 µM Hst 5 in 10 mM sodium phosphate buffer, pH 7.4, containing from 1 to 25 mM MgCl₂ (open bars) or CaCl₂ (solid bars) for 1.5 hrs at 37°C. Cell survival is expressed as percent of control and is calculated as (CFU of cells in buffer with Hst 5 + CaCl₂ or Hst 5 + MgCl₂ / CFU of cells in phosphate buffer) x 100. Each data point represents the mean ± SD of at least three independent experiments.