Figure 2. (A,B,C) Effects of doses of dentin extracts on leukocyte recruitment. Peritoneal cavities were injected with 1 mL of indicated concentrations of dentin extracts and were aspirated with PBS 6 hrs post-injection. The number of leukocytes was assessed as described in MATERIALS & METHODS. Data points represent the mean + SEM from at least 5 mice. *Significantly different from control values at P < 0.05. (D) Time-dependency of dentin-extract-induced leukocyte extravasation. Mice were treated i.p. with particulate dentin extract (D-part) (5 mg/mL) at time zero or were left untreated (control group). Peritoneal cavities were washed at the indicated time points for quantification of polymorphonuclear leukocytes (PMNs) and mononuclear cells (MNs). Data are the mean + SEM of 5 mice. (E,F) Inflammatory responses to dentin extracts in subcutaneous tissue. Mice were injected s.c. with particulate dentin extract (D-part) (5 mg/mL), and the inflammatory cell recruitment was assessed after 5 (E) and 12 days (F) (original magnifications: E, 250x; and F, 400x).