Figure 3. BMP-7-transduced keratinocytes fail to form a bone-like matrix in diffusion chambers implanted for 6 wks into c57B/6 immunocompetent BC57B/6 mice. 10⁵ BMP-7/HOKC (A), MT/HOKC (B), and BMP-7/HGF (D) were suspended in a thermoset collagen hydrogel and loaded into diffusion chambers as described (METHODS). As a control against the possibility of diffusion chamber leakage, some of the diffusion chambers were breached prior to implantation (C). The diffusion chambers were surgically implanted into subcutaneous tissue pouches in C57Bl6 mice, harvested after 6 wks, prepared for histological examination, and stained with H & E. Bar = 700 µm.