Figure 4. Effect of TNF- on CSF production, examined by anti-CSFs binding activity to lysate of cells cultured in the absence (open bar) or presence (filled bar) of TNF-. Each bar, expressing absorbance change at 415 nm per min, represents the mean value (labeled) and standard deviation of 5 determinations. Controls are shown in the left margin (cont), indicating the non-specific IgG binding activity of second antibody. *Significantly different from control (p < 0.05). **Significantly different from control, and from cells without TNF- (p < 0.005).