Figure 3. (A) Immunoblot analysis of immunoprecipitated products with anti-CSFs. In whole-cell proteins of dental pulp fibroblasts cultured without TNF-, G-CSF (19 kDa) was detected, while M-CSF (85 kDa) and GM-CSF (22 kDa) were not detected. In whole-cell proteins of dental pulp fibroblasts cultured with TNF-, G-CSF and M-CSF were detected, while GM-CSF was not detected. M1, M2 = molecular-weight markers of 19.3, 86 kDa. (B) Expressions of G-CSF, M-CSF, and GM-CSF genes. (a) Dental pulp fibroblasts. (b) VEC. PCR products for G-CSF and M-CSF mRNAs were detected in VEC at an intensity similar to that in a control. GM-CSF mRNA was detected at an intensity lower than that in VEC, and also lower than in G-CSF and M-CSF mRNAs. PCR products for ß-actin showed similar intensities in all samples.