Figure 2. Immunostaining of cultured dental pulp fibroblasts. The reaction products of anti-CSFs are visualized by green fluorescence, and of anti-Ki-67 by red fluorescence in the nuclei. (a1) G-CSF. (a2) G-CSF in IMR-90. (b) M-CSF. (c) M-CSF, with TNF-. (d) GM-CSF, with TNF-. Cells are stained by anti-G-CSF, while IMR-90 as a control was not stained. Cells are not stained by anti-M-CSF. Cells cultured with TNF- are stained by anti-M-CSF and anti-GM-CSF to a weaker extent than anti-G-CSF. Both cells in the cell-division cycle expressing Ki-67 in the nuclei and dormant cells not expressing Ki-67 are stained by anti-CSFs. There are accumulated proliferative cells (d, arrowhead). Bar = 100 µm.