Figure 2. Human alu in situ hybridization of BMSSC and DPSC transplants. (A) Human BMSSCs (dark nuclear staining) were either attached to the surface of the HA/TCP (HA) or in the connective tissue compartment (CT) at 2 wks post-transplantation. (B) After 8 wks transplantation, BMSSCs differentiated into osteoblasts (open arrows) and osteocytes (black arrows) to generate bone (B) on the surface of the HA/TCP (HA). The number of BMSSCs in the connective tissue compartment (CT) was significantly diminished. (C) Human DPSCs (dark nuclei staining) either attached to the surface of the HA/TCP (HA) or resided in the connective tissue compartment (CT) at 2 wks post-transplantation. (D) At 8 wks post-transplantation, DPSCs differentiated into odontoblasts (open arrows) to generate dentin (D) on the surface of the HA/TCP (HA). The number of DPSCs present in the connective tissue compartment (CT) is comparable with the number present at 2 wks post-transplantation. Bar, 20 µm.