Figure 3. ELISA analysis demonstrating differences in TNF-, TNF-R1, and TNF-R2 levels in TMJ tissues 48 hrs after CFA treatment. Three different samples for both CFA-treated and control were homogenized, and total protein content was determined by Lowry assays. Each sample was run in triplicate, and the results are expressed as pmol/mg protein. Data are presented as means and standard deviations and were statistically analyzed by Student’s t tests. Significance (*) was determined at a level of p 0.05.