Figure 2. Effect of CFA treatment on protein expression of TNF-, TNF-R1, and TNF-R2 in TMJ tissues. (A) Western blot analysis of TNF- and TNF receptor protein expression in TMJ tissues 48 hrs after CFA treatment. (B) The data are presented as the percent ratios of the densitometric units of the TNF-, TNF-R1, or TNF-R2 protein bands from the CFA-injected tissues to the densitometric units of the ß-actin protein band, then expressed as a percent of the uninjected controls. Data from 3 independent blots, run in triplicate, were statistically analyzed and are presented as means ± standard deviations, and significance (*) was determined at a level of p 0.05 by means of Student’s t tests.