Figure 1. Effect of CFA treatment on mRNA expression of TNF-, TNF-R1, and TNF-R2 in TMJ tissues. (A) RT-PCR of TMJ tissues demonstrating mRNA levels of TNF-, TNF-R1, and TNF-R2 after treatment with CFA for 48 hrs. ß-actin served as an internal loading control and as a means for normalization. (B) The data are presented as the percent ratios of the densitometric units of the TNF-, TNF-R1, or TNF-R2 mRNA bands to the densitometric units of the ß-actin mRNA band for the CFA-injected animals, then determined as a percent of the uninjected controls. The data are presented as the means ± standard deviations of 3 separate experiments, and significance (*) was determined at a level of p 0.05. (C) Primers and conditions used for PCR examination.