Figure 3. The cytotoxicity of 2-ethoxybenzoic acid (EBA), acetylsalicylic acid (ASA), and calcium hydroxide [Ca(OH)2] with or without the addition of eugenol (EUG) (0.1 or 0.3 mM) against human submandibular gland (HSG) cells (a) and human pulp fibroblast (HPF) cells (b). The bars indicate the means ± SD for 8 separate experiments. An asterisk denotes significance between controls and EUG by Students t test (*p < 0.05, **p < 0.01, ***p < 0.001). No significant differences between groups of EUG (0.1 and 0.3 mM) are observed in EBA, ASA, or Ca(OH)2-treated HSG cells (a). A significance in HSG cells (a) between blank and EUG is observed at 0.3 mM.