Figure 2. Effect of noggin on BMP-2-mediated expression of BSP and OCN. Cells were cultured in DMEM with 2% FBS, ± BMP-2 (300 ng/mL), ± AA (50 µg/mL), ± noggin (1 µg/mL) for 5 days, and Northern blot analysis was used to determine gene expression of BSP and OCN. Results were normalized against 18S rRNA and are shown by bar graphs. Autoradiographs are representative of results obtained in three independent experiments.