Figure 2. Binding of ³H-labeled Streptococcus gordonii DL1 to salivary proteins on polyvinylidene fluoride (A) and nitrocellulose (B) membranes. Lane 1, unstimulated whole saliva; Lane 2, experimental bracket pellicle (EBP) formed on metal brackets; Lane 3, EBP on polycrystalline alumina brackets; Lane 4, EBP on monocrystalline sapphire brackets; and Lane 5, EBP on plastic brackets. Binding of S. gordonii was mediated by acidic proline-rich proteins, a 40-kd molecule, -amylase, and low-molecular-weight mucin.