Figure 2. HIP/L29 antagonizes bFGF-induced proliferation. (A) Cells were plated as in Fig. 1. HIP/L29 (5 µg/mL) and bFGF (50 ng/mL) were added to the medium with or without 10^-9 g/mL CsA. In the control experiment, cells were cultured in MEM with 0.2% (v/v) FBS. On days 0, 3, 9, and 13, cells were trypsinized and counted. Data are presented as ± the SD of 6 experiments. *p < 0.05, **p < 0.001, ***p < 0.0001 relative to bFGF only or bFGF + CsA at day 13. (B) Cells were plated as above. The following day, cells were rinsed twice with PBS and the medium was changed to 0.2% (v/v) FBS. Cells were exposed to HIP/L29 at the indicated concentrations in the presence of 50 ng/mL bFGF. In the control experiment, gingival fibroblasts were cultured in MEM with 0.2% (v/v) FBS. Cell number was counted on days 0, 3, and 9. Data are presented as the means ± SD of 6 experiments. *p < 0.05 relative to bFGF alone at day 9.