Figure 4. Effects of 2CADO, CPA, and CGS21680 on NO production in HGEC. SV-40-transformed HGEC (1 x 10⁵/well in 24-well plates) were cultured with or without 2CADO (100 µM), CPA (50 µM), or CGS21680 (10 µM), and then NO₂^-/NO₃^- in the culture supernatants was measured as described in MATERIALS & METHODS. Results of 1 representative experiment from among 3 identical experiments are shown. Statistical analysis was performed by a one-way analysis of variance (ANOVA) with Scheffé's multiple-comparison test to a significance level of p < 0.05. Asterisks (*) indicate significant (p < 0.05) difference as compared with untreated cells.