Figure 3. Effects of CPA and CGS21680 on iNOS mRNA expression by HGEC. SV-40-transformed HGEC were cultured (1 x 10⁵/well in 24-well plates) with or without 2CADO (100 µM), CPA (50 µM), or CGS21680 (10 µM), and then RT-PCR was carried out for detection of iNOS and GAPDH mRNA expression. Results of 1 representative experiment from among 3 identical experiments are shown. The number of PCR cycles is shown above each lane. The mean ratios ± SD, which were determined as described in MATERIALS & METHODS, of iNOS mRNA expression in untreated, 2CADO-treated, CPA-treated, and CGS21680-treated epi 4 were 0.09 ± 0.15, 1.42 ± 0.35, 0.81 ± 0.26, and 0.79 ± 0.42, respectively.