Figure 3. Restriction-enzyme analysis of PCR-amplified DNA fragment of exon 4 of PAX9. The PCR fragments 450 bp long were digested with PvuII. The mutant allele was not cleaved at this site, while the wild type was cleaved normally.
Figure 3. Restriction-enzyme analysis of PCR-amplified DNA fragment of exon 4 of PAX9. The PCR fragments 450 bp long were digested with PvuII. The mutant allele was not cleaved at this site, while the wild type was cleaved normally.