Figure 2. CRAMP mRNA is expressed in the submandibular (salivary) gland and oral mucosa. CRAMP mRNA is localized by in situ hybridization with specific anti-sense CRAMP cRNA probes. Tissues were obtained from normal adult mice (A,D), newborn Day-4 mice (B,E,G-L), and fetal mice at day 17 of gestation (E17) (C,F). All submandibular glands (adult, D-4, E-17) show CRAMP mRNA expression (A-C), as do the palatine minor salivary glands (G). In the salivary glands, CRAMP mRNA expression is seen in the acinar cells, but not in ductal cells. In gingiva and palatal mucosa, CRAMP mRNA is located in the basal cell layer and a few suprabasal cell layers (H, and upper layer in I), and the epithelium of the dorsum of the tongue shows mRNA in whole epidermis (I, lower layer). Sense cRNA probes show no signal in any tissue (D-F, J-L). Original magnification: x400.