Figure 4. Laser capture RT-PCR of dentin sialoprotein (DSPP) and ß-actin mRNA in bioengineered odontoblasts. Lane 1, no template negative control; Lane 2, porcine spleen tissue; Lane 3, positive control porcine third molar odontoblasts; Lane 4, tissue-engineered (TE) odontoblasts. The DSPP product was 223 bp, and the ß-actin product was 236 bp.