Figure 3. Effects of CsA on KGFR expression by gingival epithelial cells. (A) Representative fluorescence profiles showing KGFR expression by epithelial cells cultured in the absence and presence of CsA. The dashed lines represent the fluorescence profiles of cells that received no primary antibody. The vertical black line shows the AFI of the non-treated gingival epithelial cells cultured in the absence of CsA. Note the higher AFI of the CsA-treated cells compared with the control cells, as shown in the upper righthand corner. (B) Representative experiment showing RT-PCR analysis of normal gingival epithelial cells cultured in the absence (A) and presence of CsA (B) for 3 days. Each panel shows the electrophoretic mobility of the RT-PCR products and the corresponding image analysis of these bands. Note the presence of GAPDH bands (600 bp) of similar intensities in both panels and the relatively greater amounts of the KGFR gene product (141 bp) in panel B compared with panel A. Numbers in brackets indicate the relative KGFR mRNA level in each panel.