Figure 2. RT-PCR analysis. Total RNAs from two-month-old (2M), 15-month-old (15M), and 23-month-old (23M) were reverse-transcribed. The resulting cDNAs were amplified with gene-specific primers. AA038677, Ca(2+)-transporting ATPase; U49393, sarcoendoplasmic reticulum Ca(2+) ATPase; AA024297, translocating chain-associating membrane protein; W62742, sec 61 alpha subunit; and X03672, mouse beta-actin, as a negative control. Each predicted PCR size is indicated.